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Peptide-Receptive easYmers® MHC I and U-Load® MHC II Monomers

Building blocks to easily explore the richness of immune specificity

 

Immudex is the proud global distributor of the high-quality easYmers® MHC I monomers powered by immunAware and manufacturer of U-Load MHC II.

easYmers® and U-Load MHC II are formulations of peptide-receptive MHC I and II monomers, which can be used to generate specific MHC-peptide complexes by loading your peptide of choice.

Discover U-Load® Technology

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Ultimate Flexibility, Reliable Sensitivity

Build Custom U-Load Dextramer® Reagents

Once prepared, the easYmers® or U-Load® MHC II-peptide complexes can easily be loaded onto fluorescently-labeled U-Load Dextramer® or other lab-developed MHC multimers for detection of antigen-specific CD8+ or CD4+ T cells using flow cytometry.

The Allotype Coverage to Truly Explore Human Immune Diversity

Covering a large spectrum of HLA-A, HLA-B, and HLA-C allotypes found in many world populations, the easYmers® and U-Load® MHC II monomers allow creating specific peptide-allele combinations suitable for epitope and neoepitope screening, mapping of antigen-specific T-cell responses to disease, and assessment of MHC-peptide binding.

DISCOVER THE MHC ALLELES LIST

Data obtained from Gonzalez-Galarza et al. (2020) "Allele frequency net database (AFND) 2020 update: gold-standard data classification, open access genotype data and new query tools." Nucleic Acids Res. Jan 8; 48(D1): D783–D788. Courtesy of immunAware

Discover the Step-by-Step Instructions

MHC I-Peptide Monomer and Fluorescent U-Load Dextramer® MHC I Preparation Protocol

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MHC II-Peptide Monomer and Fluorescent U-Load Dextramer® MHC II Preparation Protocol

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We can Load Your easYmers® and Assemble Your U-Load® Reagent for You!

If you're keen on an easYmers® allele but prefer not to handle the reagent production, we've got you covered. Streamline your workflow as our team takes care of loading and preparing easYmers® with your selected peptides. We assemble the U-Load® reagent, implement stringent quality control measures, and provide you with a ready-to-use, customized reagent to meet your specific requirements.

Discover our Custom Allele Services

Order easYmers® MHC I Monomers and U-Load® MHC II Monomers

Each easYmers® product contains the reagents necessary to load peptides into MHC creating easYmers® MHC I-peptide complexes, validate proper refolding of peptide-loaded easYmers® complexes via flow cytometry, as well as a protocol for the streamlined workflow to load easYmers® MHC I-peptide onto U-Load Dextramer®.

Each U-Load® MHC II product contains the reagents necessary to load peptide into MHC creating U-Load® MHC II-peptide complexes, including peptide loading component and loading buffer, as well as a protocol for the streamlined workflow to load U-Load® MHC II-peptide onto U-Load Dextramer®.

You can order easYmers® or U-Load® MHC II from the webshop below. Alternatively, please send an e-mail to [email protected] specifying your selection of easYmers® or U-Load® MHC II and your desired test size.

In case you don’t find your MHC allele of interest or other special requests then please contact us. Our Custom Solutions and Services has a very good track record of taking on custom projects and producing new alleles.

 

Can't find what you're looking for?

Our webshop does not include all products. Contact us or email [email protected] and we will search our larger internal database of products for you. We also provide custom alleles and specificities.

References

  1. Oberhardt, V., et al., Rapid and stable mobilization of CD8(+) T cells by SARS-CoV-2 mRNA vaccine. Nature, 2021.
  2. Sahin, U., et al., BNT162b2 vaccine induces neutralizing antibodies and poly-specific T cells in humans. Nature, 2021.
  3. Tippalagama, R., et al., HLA-DR Marks Recently Divided Antigen-Specific Effector CD4 T Cells in Active Tuberculosis Patients. J Immunol, 2021.
  4. Bigot, J., et al., Splicing patterns in SF3B1 mutated uveal melanoma generate shared immunogenic tumor-specific neo-epitopes. Cancer Discov, 2021.
  5. Lande, R., et al., Complementary Effects of Carbamylated and Citrullinated LL37 in Autoimmunity and Inflammation in Systemic Lupus Erythematosus. International Journal of Molecular Sciences, 2021. 22(4): p. 1650.
  6. Schulien, I., et al., Characterization of pre-existing and induced SARS-CoV-2-specific CD8+ T cells. Nature Medicine, 2021. 27(1): p. 78-85.
  7. Acevedo, G.R., et al., In Silico Guided Discovery of Novel Class I and II Trypanosoma cruzi Epitopes Recognized by T Cells from Chagas' Disease Patients. J Immunol, 2020.
  8. Azoury, M.E., et al., Peptides Derived From Insulin Granule Proteins Are Targeted by CD8(+) T Cells Across MHC Class I Restrictions in Humans and NOD Mice. Diabetes, 2020. 69(12): p. 2678-2690.
  9. Lande, R., et al., Native/citrullinated LL37-specific T-cells help autoantibody production in Systemic Lupus Erythematosus. Sci Rep, 2020. 10(1): p. 5851.
  10. Mohamed, Y.S., et al., Specificity of CD8(+) T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya. Vaccines (Basel), 2020. 8(2).
  11. Osterbye, T., et al., HLA Class II Specificity Assessed by High-Density Peptide Microarray Interactions. J Immunol, 2020. 205(1): p. 290-299.
  12. Moyo, N., et al., Efficient Induction of T Cells against Conserved HIV-1 Regions by Mosaic Vaccines Delivered as Self-Amplifying mRNA. Mol Ther Methods Clin Dev, 2019. 12: p. 32-46.
  13. Ndhlovu, Z.M., et al., Augmentation of HIV-specific T cell function by immediate treatment of hyperacute HIV-1 infection. Sci Transl Med, 2019. 11(493).
  14. Abana, C.O., et al., Cytomegalovirus (CMV) Epitope-Specific CD4(+) T Cells Are Inflated in HIV(+) CMV(+) Subjects. J Immunol, 2017. 199(9): p. 3187-3201.
  15. Nastasi, C., et al., Butyrate and propionate inhibit antigen-specific CD8(+) T cell activation by suppressing IL-12 production by antigen-presenting cells. Sci Rep, 2017. 7(1): p. 14516.
  16. Svitek, N., et al., Use of "one-pot, mix-and-read" peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle. Vet Res, 2014. 45: p. 50.
  17. Leisner, C., et al., One-pot, mix-and-read peptide-MHC tetramers. PLoS One, 2008. 3(2): p. e1678.

For research use only. Not for use in diagnostic or therapeutic procedures.

The easYmers® technology is powered by immunAware.