Dextramer® Controls – Best Practices in Research Call for the Best Controls in Experiments

Controls eliminate confounding factors, distinguish signal from background, and help troubleshoot protocols

Controls Are the Core of Every Experiment

Positive and negative controls are often an afterthought in the design of experiments. And yet, without them, results leave room for ambiguity. Only properly controlled experiments can concretely discriminate hypotheses.

Controls also help resolve technical issues in experiments:

Dextramer® Negative Controls help maximize signal-to-noise ratios and enable accurate monitoring of your positive cell population.
Dextramer® Positive Controls help optimize the staining procedure without wasting time, effort, and resources.

MHC I Dextramer® Negative Control staining allows to distinguish a MART-1-specific CD8+ T-cell population from background staining using flow cytometry.

Order Dextramer® Controls

Couple your Dextramer® reagents with the right positive and negative controls. Immudex offers different options to design Dextramer® Positive and Negative Controls with fluorophore labelling (PE, APC, and FITC) for your flow cytometry and immunohistochemistry experiments. See the allele and antigen combinations we propose for Positive and Negative controls.

To order, send an email to [email protected] specifying:

Catalog number or desired MHC allele-peptide combination
Fluorophore (PE, APC, FITC or none)
Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 10⁶ lymphid cells or 1–3 x 10⁵ clonal antigen-specific T cells

Dextramer® Controls

Depending on your experimental setup, you can order Dextramer^® Negative Controls carrying:

a non-sense peptide sequences for Human MHC I allele
a peptide sequence unrelated to your experiment
a CLIP peptide for Human MHC II allele

Depending on your experimental setup, you can order Dextramer^® Positive Controls that:

recognize epitopes derived from widespread human viruses (CMV, EBV, and Flu)
carry three viral epitopes from CMV, EBV and Flu and are then pooled

*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, in OVA mouse models, it can serve as a negative control reagent, while H-2KB/SIINFEKL is the positive control reagent [1,2].

*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, this peptide is expressed in certain mouse models for tumors and recognized by CD8+ T cells expressing 2C TCR. Thus, it can be used as a positive control [4,5].

Can’t find what you’re looking for?

Our webshop does not include all products. Contact us or email [email protected] and we will search our larger internal database of products for you. We also provide custom alleles and specificities.

Add a custom product - MHC I

Allele	Peptide	Dye	Size	Cat. No.	Price	Buy
				CUSTOM-01	$1,540.00 $2,730.00 On request On request

Add a custom product - MHC II

Allele	Peptide	Dye	Size	Cat. No.	Price	Buy
				CUSTOM-02	$1,700.00 $3,295.00 On request On request

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Dextramer® Custom Controls

Your experimental setup may require an MHC allele that is not listed in our catalog or different specificities. Our Custom Solutions and Services Team can ensure you have the right controls for your research. Just contact us.